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The effects of the sfr2, sfr3 and sfr6 mutations on lyotropic stress responses-- cold damage repair

Experiment Name: The effects of the sfr2, sfr3 and sfr6 mutations on lyotropic stress responses-- cold damage repair
Accession No. & GeneChip: AT20, ATH1-121501
Submitter: Experimenter: Dr Gareth Warren, submitted by Lishuang Shen
Experiment Type: genotype
stimulus or stress
Experiment Factor: time
Number of Replicates: 2
Quality Control Steps: biological replicates
Quality Control Description: None
Publication_id: None
Last Update Time: 2004-11-03 17:08:26
Expression Data
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Description: This experiment submission is a courtesy of the Nottingham Arabidopsis Stock Centre's microarray database (http://ssbdjc2.nottingham.ac.uk/narrays/experimentbrowse.pl).The data was generated from NASC's Affymetrix service and offered for public access at BarleyBase.

If you publish with this data, please reference data from NASC/GARNet:

Craigon DJ., James N., Okyere J., Higgins J., Jotham J., May S.
NASCArrays: A repository for Microarray Data generated by NASC's Transcriptomics Service.
Nucleic Acids Research, (2004). volume 32, Database issue D575-D577.

Following is the original experiment description from NASCArray:
Experiment: The effects of the sfr2, sfr3 and sfr6 mutations on lyotropic stress responses
Submitted to this database: 24/10/2003

Browse this folder on CD

Experiment Description

Our goals are to discover the basis of the stress-sensitive phenotypes of the sfr2, sfr3 and sfr6 mutants, and to distinguish damage-repair from damage-prevention-related transcription in the wild type.

Part I. The effects of sfr2 and sfr3 on cold-induced gene expression will be observed. Since sfr6 causes sensitivity to drought as well as freezing, the effects of sfr6 on the transcriptional response to drought is studied; an observation of cold-induced sfr6 expression is needed for direct comparison to the effect of drought. Unstressed mutants, and equivalently-stressed wild types, are necessary controls. The above experiments are conducted on tissue-culture-grown plants grown under 24 hr illumination for maximum reproducibility and comparability to other transcriptomic experiments.

II. Freezing causes physiological changes even in a hardy, cold-acclimated wild type. During the recovery period, gene expression will reflect the induction of damage-repair processes distinct from the damage-prevention associated with cold acclimation. This will be detected by observing the wild-type transcriptome at two time points during recovery from a freezing episode. The appropriate controls is the unfrozen, cold-acclimated wild type. Plants for this experiment will be soil-grown in an 9/15 day/night cycle to the rosette stage, and this regime will be maintained during acclimation and freezing treatments.Cold-induction will be 10 days at 4°C; drought will be imposed by placing excised leaves in a desiccator for 6 h. The youngest fully-expanded rosette leaves will be harvested for RNA extraction.

This is the part II of this experiment.

About the Experimenter
Name: Dr Gareth Warren
Head of Lab Name: Dr Gareth Warren
Lab:
Address: Biological Sciences
Royal Holloway
University of London
Egham
Surrey

Postcode: TW20 0EX
Country: United Kingdom
Telephone Number: 01784-443778
Fax Number: 01784-470756

About this Experiment
Experiment Type: stress response
.
Experimental Parameters:
parameter time-course
parameter mutant
parameter treatment
Quality Control Measures Taken:
no-plants-pooled 18

All of the data available in this website/database is free, and you are free to do whatever you please with it. If you intend to publish work based on any of this data, please acknowledge us, contact the experimenter above, and either acknowledge them or use them as co-authors in the work.
Providers:
Name: Dr Gareth Warren
Institution: Biological Sciences Royal Holloway University of London Egham S
Head of Laborotary: Dr Gareth Warren
email:
Homepage:

 
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