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Carbohydrate- and redox-regulation of gene expression in a TPT mutant | ||||
| Experiment Name: | Carbohydrate- and redox-regulation of gene expression in a TPT mutant | |||
| Accession No. & GeneChip: | AT16, ATH1-121501 | |||
| Submitter: | Experimenter: Robin Walters, submitted by Lishuang Shen | |||
| Experiment Type: | dose response effect of gene knock-out | |||
| Experiment Factor: | dose genotype | |||
| Number of Replicates: | 3 | |||
| Quality Control Steps: | biological replicates | |||
| Quality Control Description: | None | |||
| Publication_id: | None | |||
| Last Update Time: | 2004-06-10 09:30:30 | |||
| Expression Data Access & Analysis: | Please select one action: 1. Batch download all data files at Download Center . 2. Browse Hybridizations and boxplots from experiment. 3. Visualization of hybridizations or treatment means with scatterplots and MvA plots. 4. Browse Samples from experiment. 5. Create Gene List by filter probe sets for differentially-expressed genes. 6. Pattern Recognition on filtered gene list. | |||
| Description: | This experiment submission is a courtesy of the Nottingham Arabidopsis Stock Centre's microarray database (http://ssbdjc2.nottingham.ac.uk/narrays/experimentbrowse.pl).The data was generated from NASC's Affymetrix service and offered for public access at BarleyBase. If you publish with this data, please reference data from NASC/GARNet: Craigon DJ., James N., Okyere J., Higgins J., Jotham J., May S. NASCArrays: A repository for Microarray Data generated by NASC's Transcriptomics Service. Nucleic Acids Research, (2004). volume 32, Database issue D575-D577. Following is the original experiment description from NASCArray: About the Experimenter Name: Dr Robin Walters Head of Lab Name: Dr Nicholas Kruger Address: Dept of Plant Sciences University of Oxford South Parks Road Oxford Postcode: OX1 3RB Country: UK Telephone Number: 01865 275000 Fax Number: 01865 275074 Experiment: Carbohydrate- and redox-regulation of gene expression in a TPT mutant Submitted to this database: 27/10/2003 Experiment Description The triose-phosphate/phosphate translocator (TPT) of the chloroplast inner envelope membrane mediates the counter-exchange of stromal triose phosphates derived from CO2 fixation with cytosolic phosphate, thus providing the cytosol with precursors for sucrose synthesis. We have isolated an Arabidopsis mutant (tpt-1) in which the gene encoding TPT is disrupted by a T-DNA insertion. During growth in low light tpt-1 plants are phenotypically normal, but in high light photosynthesis is inhibited and growth is retarded relative to wildtype. This mutant compensates for the absence of TPT by diverting photosynthate into starch which is hydrolysed and exported from the chloroplast as glucose that is subsequently phosphorylated by hexokinase. In low light the capacity of the pathway of starch synthesis is sufficient to accommodate the normal rate of CO2 fixation, but in high light it is unable to match the potential rate of CO2 fixation. Consequently, in high light-grown plants there are measurable effects on the redoxstate of several components. Thus, the tpt-1 mutation influences the carbohydrate status within the cell, alters the form in which carbon is received by the cytosol, and changes the redox signals that are important in photosynthetic acclimation.Plants will be grown in a 8h light:16h dark regime at both 400 and 100 µmol PAR m-2 s-1. Total RNA will be extracted from pools of individual illuminated leaves from at least eight plants at growth stage 3.70. Leaves will be harvested 2 h into the photoperiod to maximises differences between plant lines in the expression of genes of photosynthesis and carbohydrate metabolism. We anticipate that expression of many genes will be affected by the absence of TPT and by changes in light intensity. However, by comparing differences in transcript levels between wildtype and TPT mutant grown in high light with the differences that occur in plants grown in low light we will discriminate between genes whose expression is affected by changes in the pattern of carbohydrate metabolism and those influenced by redox poise of the thylakoid photochemical components. These comparisons will also highlight genes affected by recently identified regulatory interactions between sugar-sensing and redox-sensing. A genome-wide expression study will establish the extent to which gene expression is altered by the absence of TPT in leaves, and will provide the basis for more detailed analysis of a selected range of transcripts whose levels of expression differ between plant lines. About this Experiment Experiment Type: light treatment Experimental Parameters: parameter Mutant/Wildtype Quality Control Measures Taken: no-plants-pooled 8-12 biological-replicates 3 All of the data available in this website/database is free, and you are free to do whatever you please with it. If you intend to publish work based on any of this data, please acknowledge us, contact the experimenter above, and either acknowledge them or use them as co-authors in the work. | |||
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