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Analysis of anther development by analysing downstream genes controlled by MS1 | ||||
| Experiment Name: | Analysis of anther development by analysing downstream genes controlled by MS1 | |||
| Accession No. & GeneChip: | AT15, ATH1-121501 | |||
| Submitter: | Experimenter: Ms Gema Vizcay Barrena, submitted by Lishuang Shen | |||
| Experiment Type: | Male sterility | |||
| Experiment Factor: | genotype tissue type | |||
| Number of Replicates: | 3 | |||
| Quality Control Steps: | biological replicates | |||
| Quality Control Description: | None | |||
| Publication_id: | None | |||
| Last Update Time: | 2004-06-10 09:33:24 | |||
| Expression Data Access & Analysis: | Please select one action: 1. Batch download all data files at Download Center . 2. Browse Hybridizations and boxplots from experiment. 3. Visualization of hybridizations or treatment means with scatterplots and MvA plots. 4. Browse Samples from experiment. 5. Create Gene List by filter probe sets for differentially-expressed genes. 6. Pattern Recognition on filtered gene list. | |||
| Description: | This experiment submission is a courtesy of the Nottingham Arabidopsis Stock Centre's microarray database (http://ssbdjc2.nottingham.ac.uk/narrays/experimentbrowse.pl).The data was generated from NASC's Affymetrix service and offered for public access at BarleyBase. If you publish with this data, please reference data from NASC/GARNet: Craigon DJ., James N., Okyere J., Higgins J., Jotham J., May S. NASCArrays: A repository for Microarray Data generated by NASC's Transcriptomics Service. Nucleic Acids Research, (2004). volume 32, Database issue D575-D577. Following is the original experiment description: About the Experimenter Name: Ms Gema Vizcay Barrena Head of Lab Name: Dr Zoe Wilson Address: School of Biosciences University of Nottingham Sutton Bonington Loughborough Leics Postcode: LE12 5RD Country: UK Telephone Number: 0115 9513235 Fax Number: 0115 9516334 Experiment: Analysis of anther development by analysing downstream genes controlled by MS1 Submitted to this database: 23/10/2003 Experiment Description We have cloned the Arabidopsis MALE STERILITY1 (MS1) gene and shown it to have homology to the PHD-finger class of transcriptional regulators (Wilson et al 2001 Plant J. 28: 27-39). The overall importance of MS1 in pollen development has been clearly demonstrated since when mutated pollen degenerates after microspore release leaving a male sterile but otherwise phenotypically normal plant. We have demonstrated that MS1 is expressed in the tapetum around the stage of microspore release prior to tapetal degeneration. Staging of buds will be defined by bud size. Comparisons of gene expression will be made between the Ler-0 wt and ms1 mutant to identifying changes in expression patterns of genes of critical importance. The identified genes will then be analysed for their expression patterns by RT-PCR and sequence/database homology to identify possible functions. Reverse genetics will be used to obtain the corresponding mutants by hybridising DNA filters of the SLAT and Gene Machine-derived transposon populations with the "identified genes". In situ hybridisations will be conducted using the newly identified geneson wild type and our collection of male sterile mutants to characterise and co-ordinate the patterns of gene expression during anther development. About this Experiment Experiment Type: Male sterility Experimental Parameters: Parameter Mutant (MS1) / Wildtype Parameter Flower Bud Size Quality Control Measures Taken: no-plants-pooled 20 minimum All of the data available in this website/database is free, and you are free to do whatever you please with it. If you intend to publish work based on any of this data, please acknowledge us, contact the experimenter above, and either acknowledge them or use them as co-authors in the work. | |||
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