| Hello! Guest! Please Login or Register! | Log Out |
Arabidopsis thaliana gene expression during floral transition and early flower development | ||||
| Experiment Name: | Arabidopsis thaliana gene expression during floral transition and early flower development | |||
| Accession No. & GeneChip: | AT4, ATH1-121501 | |||
| Submitter: | Experimenter: MPI Tübingen, submitted by Lishuang Shen | |||
| Experiment Type: | development or differentiation | |||
| Experiment Factor: | genotype organism part developmental stage | |||
| Number of Replicates: | 2 | |||
| Quality Control Steps: | biological replicates | |||
| Quality Control Description: | None | |||
| Publication_id: | None | |||
| Last Update Time: | 2004-06-10 16:49:26 | |||
| Expression Data Access & Analysis: | Please select one action: 1. Batch download all data files at Download Center . 2. Browse Hybridizations and boxplots from experiment. 3. Visualization of hybridizations or treatment means with scatterplots and MvA plots. 4. Browse Samples from experiment. 5. Create Gene List by filter probe sets for differentially-expressed genes. 6. Pattern Recognition on filtered gene list. | |||
| Description: | This experiment data was first submitted to TAIR, and is provided by TAIR for public access. Coordinator: Lutz Nover, Detlef Weigel and Thomas Altmann. The details about the AtGenExpress project can be found at: http://www.uni-frankfurt.de/fb15/botanik/mcb/AFGN/atgenex.htm TAIR's Original Overview About the experiment: "The MPI Tübingen (Schmid, Lohmann and Weigel labs) have supplied data from 280 Affymetrix arrays, representing 100 experimental treatments." "This is part of the large collaborative project, AtGenExpress. AtGenExpress was proposed by the German DFG's AFGN (http://www.uni-frankfurt.de/fb15/botanik/mcb/AFGN/AFGNHome.html) project, the US NSF , RIKEN , GARNet, and several smaller groups that will combine resources to use microarray technology to profile gene expression patterns across developmental time and in response to hormones and stress." "Floral transition and flower development are regulated by numerous environmental and endogenous signals, which are integrated at a relatively small number of floral integrators, such as FLOWERING LOCUS T (FT) and SUPPRESSOR OF CONSTANS OVEREXPRESSION 1 (SOC1). Of the environmental factors, photoperiod is regarded the most important one in promoting floral transition in Arabidopsis thaliana and most labstrains will flower earlier under long day (LD) conditions than under short day (SD) conditions. Arabidopsis is therefore considered a facultative LD plant. To monitor gene expression changes during floral transition and early flower development plants were grown under SD (9 hr light, 15 hr dark) for 30 days. Plants were then shifted to LD (16 hr light, 8 hr dark) conditions to induce flowering. RNA was isolated from micro-dissected apical tissue harvested 0, 3, 5, and 7 days after the shift to LD and double-stranded cDNA was synthesized. Biotinylated cRNA probes were prepared and hybridized to the Affymetrix ATH1 array in duplicate (biological replicates). To study floral transition, we not only analyzed response of wildtype Landsberg erecta (Ler) plants, but also the effect of mutants in the flowering time genes CONSTANS (CO; co-2) and FT (ft-2). Early flower development was analyzed by comparing Col-0 wildtype plants with the meristem identity mutant lfy-12 (Col-0)." BarleyBase downloaded data from TAIR and make our own processing for public query. Based on the experiment description, we splitted the data set into 2 experiment submissions in BarleyBase: 40 arrays are stored in this experiment (BB17), and another 240 arrays stored in another experiment, BB18. | |||
Providers: | ||||
|
|
||
| Copyright@2001-2003 The BarleyBase Group All rights reserved. |
For problems with the webpages, contact
barleybasewebmaster |
|